|
KMID : 0616120210520010034
|
|
Korean Journal of Pharmacognosy
2021 Volume.52 No. 1 p.34 ~ p.40
|
|
|
The Mechanism of Whole Plant Extract of Viola verecunda on the Proliferation of Dermal Papilla Cells
|
|
Kang Jung-Il
Seo Min-Jeong
Choi Youn-Kyung
Shin Su-Young
Hwang Yong
Goh Jae-Duk
Yoo Eun-Sook
Kim Sang-Cheol
Kang Hee-Kyoung
|
|
|
Abstract
|
|
|
|
Proliferation and maintain of dermal papilla during progression of hair-cycle are crucial to the duration of anagen and regulated by diverse signaling pathway such as PI3K/Akt/Wnt/β-catenin pathway. In this study, we investigated the effects and mechanisms of Viola verecunda on dermal papilla cells. Treatment of dermal papilla cells with whole plant extract of V. verecunda resulted in cell proliferation, which was accompanied by up-regulation of cyclin D1, phospho (ser780)-pRB and cdc2 p34, and down-regulation of p27kip1. V. verecunda extract also promoted the levels of phospho (ser473)-Akt and phospho (ser780)-pRB in a time-dependent manner. Inhibition of PI3K/Akt by Wortmannin suppressed progression of cell-cycle, thereby attenuated the increases in proliferation of dermal papilla cells by V. verecunda extract. We further investigated Wnt/β-catenin pathway with respect to the effects of V. verecunda extract on the proliferation of dermal papilla cells. Treatment with V. verecunda extract results in up-regulation of Wnt/β-catenin proteins such as phospho (ser9)-GSKβ, phospho (ser552)-β-catenin and phospho (ser675)-β-catenin. In addition, Wortmannin abrogated V. verecunda extract mediated up-regulation of cdc2 p34 and down-regulation of p27kip1. These finding reveal that the proliferative effect of V. verecunda mediated by alteration of cell-cycle via activating PI3K/Akt/Wnt pathway in dermal papilla cells.
|
|
|
KEYWORD
|
|
|
Viola verecunda, Dermal papilla cells, PI3K/Akt, Wnt/β-catenin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|